National Center for Macromolecular Imaging

NCMI Major Equipment


NCMI's major equipment includes :
  • 8 Gatan cryo-specimen holders for three of the JEOL 200 keV electron microscopes.
  • 2 FEI Vitrobot vitrification robots used for cryo-specimen preparation.
  • 2 Gatan cryo-plunger (CP3) used for cryo-specimen preparation.
  • 1 Leica EMGP automatic plunge freezing apparatus.
  • Advanced Gatan and Fishione plasma cleaning systems for treating EM-grids and other devices.
  • 2 EMITech carbon evaporators.
  • 3 Light microscopes from Nikon and Olympus; One Nikon Fluorescent microscope.
  • Linkam's CMS196 cryo-light microscope stage.
  • Instec's CLM77K cryo-light microscope stage.
  • Leica High Pressure Freezer (EM PACT2); Leica automated freeze substitution unit and Leica cryo-Ultramicrotome (EM UC6) with cryo-sphere.
  • Tissue culture laboratory equipped with Thermo Scientific Heraus Megafuge16R, Ohaus Scout Pro scale, Orflo MaxiZ Mini Automated Cell Counter, Nuaire Biological Safety Cabinet Class II Type A/B3 Model Nu-425-400, Nuaire Autoflow IR Direct Heat CO2 Incubator (2x), VWR Fridge (Full Size and Mini), VWR Liquid Nitrogen Storage, VWR Waterbath and VWR Analog Vortex Mixer.
  • IDOME for immersive 3-D visualization.
  • Tissue Culture Room

    Tissue culture laboratory equipped with Thermo Scientific Heraus Megafuge16R, Ohaus Scout Pro scale, Orflo MaxiZ Mini Automated Cell Counter, Nuaire Biological Safety Cabinet Class II Type A/B3 Model Nu-425-400, Nuaire Autoflow IR Direct Heat CO2 Incubator (2x), VWR Fridge (Full Size and Mini), VWR Liquid Nitrogen Storage, VWR Waterbath and VWR Analog Vortex Mixer



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    Leica EM PACT2(high pressure freezing)

    Leica High Pressure Freezer (EM PACT2); Leica automated freeze substitution unit and Leica cryo-Ultramicrotome (EM UC6) with cry-sphere.



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    Cryo-sectioner



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    Cryo-LM



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    Carbon evaporator

    2 EMITech carbon evaporators.



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    Plasma cleaner

    Advanced Gatan and Fishione plasma cleaning systems for treating EM-grids and other devices.



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    Glow Discharge Cleaning System

    PELCO easiGlow



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    Dry pumping station



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    Gatan_cp3

    2 Gatan cryo-plunger (CP3) used for cryo-specimen preparation.



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    Leica-EMGP

    1 Leica EMGP automatic plunge freezing apparatus.



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    Vitrobot Mark IV

    2 FEI Vitrobot vitrification robots used for cryo-specimen preparation.



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    Other equipment:

  • 3 Light microscopes from Nikon and Olympus.
  • One Nikon Fluorescent microscope.
  • Linkam's CMS196 cryo-light microscope stage.
  • Instec's CLM77K cryo-light microscope stage.