National Center for Macromolecular Imaging
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Structural & Computational Biomedical Informatics and Cryo-EM (United Kingdom)

Cryo-EM Workshop Single particle reconstruction with and without symmetry National Institute of Biological Standards and Control South Mimms, Potters Bar, Herts, UK June 8-12, 2009
Workshop Objectives: 1. Practical on high quality and computer-assisted data collection of ice-embedded specimens 2. Single particle reconstruction with and without symmetry 3. Tomographic reconstruction and post-tomographic averaging of sub-tomograms 4. Structure mining of cryo-EM density map 5. Training on latest update to EMAN2, �working without symmetry� Workshop Agenda: Day 1 - Morning � Wah Chiu : How to get high quality images of ice embedded specimens � Htet Khant : What to do and not to do in cryo-specimen preparation Day 1 - Afternoon � Cryo-EM sample preparation, low dose imaging, cryo-TEM Day 2 - Morning � Computer assisted data collection (JADAS) � Steve Ludtke: Introduction to Single Particle Reconstruction Day 2 - Afternoon � EMAN2 Single particle reconstruction tutorial Day 3 - Morning � Wen Jiang : Icosahedral and asymmetric reconstructions of viral particles � Wah Chiu: Integration of cryo-EM maps, bioinformatics and modeling Day 3 - Afternoon � Wen Jiang: Symmetric and asymmetric reconstruction of icosahedral particles using EMAN Day 4 - Morning � David Mastronade: 3D tomography � Tomographic reconstruction using IMOD Day 4 - Afternoon � Michael Schmid: Retrieving features by post-tomographic averaging of subtomograms � Post-tomographic averaging using EMAN2 Day 5 - Morning Practical�s � Expert Panel discussions � Opportunity for students to focus on specific practical training Morning and afternoon symposium with speakers to be announced