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Structural & Computational Biomedical Informatics and Cryo-EM (United Kingdom)
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Cryo-EM Workshop
Single particle reconstruction with and without symmetry
National Institute of Biological Standards and Control
South Mimms, Potters Bar, Herts, UK
June 8-12, 2009
Workshop Objectives:
1. Practical on high quality and computer-assisted data collection of ice-embedded specimens
2. Single particle reconstruction with and without symmetry
3. Tomographic reconstruction and post-tomographic averaging of sub-tomograms
4. Structure mining of cryo-EM density map
5. Training on latest update to EMAN2, “working without symmetry”
Workshop Agenda:
Day 1 - Morning
• Wah Chiu : How to get high quality images of ice embedded specimens
• Htet Khant : What to do and not to do in cryo-specimen preparation
Day 1 - Afternoon
• Cryo-EM sample preparation, low dose imaging, cryo-TEM
Day 2 - Morning
• Computer assisted data collection (JADAS)
• Steve Ludtke: Introduction to Single Particle Reconstruction
Day 2 - Afternoon
• EMAN2 Single particle reconstruction tutorial
Day 3 - Morning
• Wen Jiang : Icosahedral and asymmetric reconstructions of viral particles
• Wah Chiu: Integration of cryo-EM maps, bioinformatics and modeling
Day 3 - Afternoon
• Wen Jiang: Symmetric and asymmetric reconstruction of icosahedral particles using EMAN
Day 4 - Morning
• David Mastronade: 3D tomography
• Tomographic reconstruction using IMOD
Day 4 - Afternoon
• Michael Schmid: Retrieving features by post-tomographic averaging of
subtomograms
• Post-tomographic averaging using EMAN2
Day 5 - Morning
Practical’s
• Expert Panel discussions
• Opportunity for students to focus on specific practical training
Morning and afternoon symposium with speakers to be announced
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