National Center for Macromolecular Imaging
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Cryo-EM Workshop: Cryo Specimen Preparation


DATE
Oct.  25, 2010 - Oct. 29, 2010 
10am-5pm

LOCATION
N420 Alkek
Baylor College of Medicine
Houston, TX 77030

Instructor
SPEAKERS

Htet Khant Caroline Fu
NCMI NCMI
 

ORGANIZERS

Htet Khant Wah Chiu
hkhant@bcm.edu wah@bcm.edu
*administrative contact  
 
The workshop will focus on practical aspects of vitrification using FEI's Vitrobot Mark IV and Gatan Cryoplunge 3. Participants will prepare lipid vesicles and individually workout appropriate conditions to successfully vitrify the sample. Both sample preparation and the vitrification conditions need to be optimized in such a way that the lipid vesicles are uniformly dispersed in a thin film of amorphous ice with minimal contamination.

Vitrified samples prepared by participants will be imaged on JEM2100 cryo electron microscope using Gatan's 626 cryo-holder. Basic alignment procedure and low dose setup on the microscope will be briefly introduced. Participants will also learn how to screen samples using "manual mode" as well as automated data collection software package (JADAS-JEOL Automated Data Acquisition System).

Registration for the workshop is now closed.