Present Research

Determination of  3-Dimensional structure of AQP1 at 3.7A  resolution.
The existing 3-dimensional data set have be enhanced by recording high-resolutionm hight-tilt images and electron diffraction patterns. For this purpose, modern techniques of specimen preparation, data acquisition and data analysis will be followed to generate data to progressively higher resolution. The ultimate goal is to determine the path of the polypeptide chain in the 3-D structure from which a chemical model for the regulation of water transport can be postulated. I found that the flatness of molybdenum grids for 2-D crystals is critical important. we thanks Pacific GridTech (Pacific Grid-Tech) provide us free molybdenum grids for this research work. The reason can be find by click following link, here.
Examine, at the structural level, the pharmacological inhibition of water transport in AQP1 by mercurial compounds.
Mercurial compounds reversiblly inhibit water transport in AQP1 by binding to an extracellular cysteine (CYS189) residue in the sequence. A preliminary difference Fourier map in projection has be calculated by using data recorded from AQP1 crystals labeled with the mercurial reagent pCMBS. The extended study is doing by recording and analyzing diffraction patterns from the tilted crystals. This will allow us to understand the structural correlates of inhibition of water transport by mercurial compounds and the location of the aqueous pathway in 3-dimensions.

Measurement Structure Factors from CBED Patterns
In the latest decade, the development of electron microscopes which can obtain digitally collected energy-filtered diffraction patterns coupled with increasing computer power, has made the matching of theoretical calculations to experimental energy-filtered convergent beam electron diffraction (CBED) patterns a possibility. There has been a growing realization over the past few years that the matching of experiment and computed patterns has the potential to expand greatly the scope of quantitative CBED. Using the refinement of very accurate low-order structure factors in known crystal structures, it is possible to determine the valence charge distribution in the crystal and address questions such as the nature of the bonding, bond charge, ionicity etc.
Computation and Image Process
Making programs to measurement the modulation transfer function of SSCCD and restoration scheme using the deconvolution of the experimentally recorded raw elastic CBED patterns, and accurate measurement of the spherical aberration coefficient of Philips CM200-FEG using SSCCD micrographs.
TEM Studying Electron Function Materials
1. The Melting Behavior of In Nanoparticles Embedded in Al Matrices; The Melting Behavior of Pb Nanoparticles Embedded in Al Matrices.
2.The Annealing Temperature Dependence of the Grain Size of BaTiO3 Thin Films Prepared by Sol-Gel Method