Useful Links

 

3D Electron Microscopy Labs

  • The Boulder Laboratory for 3-Dimensional Fine Structure, Boulder, Colorado, USA 

  • Division of Structural Biology, University of Oxford, England 

  • The Centre for Biomolecular Electron Microscope, Imperial College, London, England 

  • Department of Molecular Structural Biology, MPI, Martinsried, Germany 

  • The National Center for Microscopy and Imaging Research in San Diego, CA, USA 

  • The National Center for Macromolecular Imaging, Baylor College of Medicine, Houston, Texas, USA 

  • The M.E. Mueller-Institute for Structural Biology at the Biozentrum, Basel. 

  • The Purdue University Cryo- Electron Microscopy and Image Analysis Laboratory, USA 

  • Centre for Microscopy & Microanalysis, The University of Queensland, Australia 

  • Resource for the Visualization of Biological Complexity at the Wadsworth Center, Albany, NY 


    •    3D Tomography group of the Department for Electron Microscopical Structural Analysis, Universiteit Utrecht, The    Netherlands 

    Packages for 3D-EM Image Analysis

  • AUTO/EDP

  • National Center for Microscopy and Imaging Research 

  • EMBL: Icosahedral Program Documentation

  • The Eos System

  • EMAN

  • The IMAGIC Package 

  • Interactive Crystallographic Environment (ICE) 

  • IMOD

  • The NIH Image Home Page

  • PHOELIX 

  • PTOOL

  • The Micrograph Data Processing Program (MDPP). 

  • The "Situs" Package

  • The Spider and Web Packages. 

  • The Stereo Contour Reconstruction System for Microscopy Data (STERECON) 

  • The XMIPP Package.

    • Other Information Resources

  • Look at the The 3D-EM Database Prototypes: 

  • The Microscope 3D database (from WCLR Albany NY) 

  • The Microscope 3D database (from CNB Spain) 


    • The 3D Reconstruction Web Site 

    High Quality Grids Provider for  Cryo-EM

    Pacific Grid-Tech (Pacific GridTech), a high quality TEM grid provider. Grids with very smooth surface are strongly recommended for two-dimensional crystallography, which is evidenced in many peer reviewed articles. such as

    "Measurements on grids made of titanium, molybdenum and tungsten showed significantly less puckering."

    -- Booy F.P. etc., Cryo-crinkling: what happens to carbon films on copper grids at low temperature. Ultramicroscopy. 48(1993)3,  273-80

    "... the use of molybdenum grids has become standard in electron cryo-microscopy. However, ... the rough surface of these grids can also induce the carbon film to crinkle"

    -- Fujiyoshi Y., The structural study of membrane proteins by electron crystallography, Adv. Biophysics, 35(1998) 25-80.

    "One of the main problems in data collection of 2D protein crystals for EM is a lack of flatness of the specimens. The resulting overlap of data from crystal areas of slightly different tilt angles (visible as blurring of the diffraction spots in the direction perpendicular to the tilt axis) is especially severe at high tilt angles and effectively reduces the vertical resolution of a 3-D map."

    -- Vonck, J., Parameters affecting specimen flatness of two-dimensional crystals for electron crystallography, Ultramicroscopy, 5(2000) 123-129.

    I found that the best grids for cryocrystallography is 300 mesh molybdenum grids (Mo grids) provided by Pacific GridTech (Pacific Grid-Tech).

     

    Pacific GridTech

    http://www.grid-tech.com/

    Email: info@grid-tech.com/

    1