Welcome To The JAMES FAQ
What is my angstrom/pixel?
While this isn't really a JAMES question, it gets asked alot by people using JAMES so I will answer it here.
angstrom/pixel = digitization step size / magnification
For any CCD the digitization step size is equal to the pixel size of the detector. Contact your manufacturer to find the pixel size of your camera. One thing to keep in mind, is that the detectors often sit in a different location from film in the microscope. This means that the effective magnification on the detector is different from the readout on your microscope. This value will have to be calibrated, you can do this yourself or have the microscope engineers do this for you.
For NCMI users, this magnification factor using the CCD on the 2010F is 1.38, on the 3000SFF it is 1.41, both CCDs have a pixel size of 15 µm or 150,000 Å. So to calculate the angstrom/pixel value on the 3000SFF:
angstrom/pixel = 150,000 Å / (microscope magnification x 1.41)
For a microscope magnification of 60,000 on the 3000SFF:
angstrom/pixel = 150,000 Å / (60,000 x 1.41)
angstrom/pixel = 1.77
