Curriculum Vitea

Jaap Brink, Ph.D.

Address:: W.M. Keck Center for Computational Biology and; Verna and Marrs McLean Department of Biochemistry; Baylor College of Medicine; One Baylor Plaza; Houston, Texas 77030; Telephone: (713) 798-6989; FAX: (713) 796-9438; E-mail: jbrink@bcm.tmc.edu

Education:: B.Sc. 1979, Rijksuniversiteit Groningen, The Netherlands (Biochemistry); M.Sc. 1983, Rijksuniversiteit Groningen, The Netherlands (Biochemistry); Ph.D. 1988, Rijksuniversiteit Groningen, The Netherlands (Biochemistry)

Societies:: Electron Microscopy Society of America (EMSA)

Professional Experience:: 1988 - 1990 Research Associate, Biochemistry Department, Baylor College of Medicine; 1990 - W.M. Keck Postdoctoral Fellow, Biochemistry Department, Baylor College of Medicine

Meetings:

Speaker at 47th EMSA meeting 1989 in San Antonio, TX
Invited speaker at XIIth ICEM meeting 1990 in Seattle, WA
Speaker at 10th EUREM 1992 in Granada, Spain
Invited speaker at mini-workshop 'DigitalMicrograph' 1992 held by Gatan in Pleasanton, CA
Panel discussion member Gordon Research Conference '3-Dimensional Electron Microscopy of Macromolecules' in 1993 held at New Hampton School, NH
Speaker at workshop 'Molecular Imaging of the Cytoskeletal Protein Assembly' 1994 held at Baylor College of Medicine, Houston, TX
Speaker at 52nd MSA meeting 1994 in New Orleans, LA.
Invited speaker at 53rd MSA meeting 1995 in Kansas City, MI.
Workshop Instructor at the 'Cryo-electron Microscopy Workshop' at Purdue University, Nov. 3-6, 1996
Invited speaker at the 1988 ICEM meeting in Cancun, Mexico.

Regular papers

Roossien, F.F., Brink, J. and Robillard, G.T. (1983) A simple procedure for the synthesis of [³²P]phospho-enol-pyruvate via the pyruvate kinase exchange reaction at equilibrium, Biochim. Biophys. Acta 760:185-187.
Brink, J., Hovmöller, S., Ragan, C.I., Cleeter, M.W.J., Boekema, E.J. and Van Bruggen, E.F.J. (1987) The structure of NADH:ubiquinone oxidoreductase from beef-heart mitochondria. Crystals containing an octameric arrangement of iron-sulphur protein fragments, Eur. J. Biochem. 166:287-294.
Brink, J., Boekema, E.J. and Van Bruggen, E.F.J. (1988) Electron microscopy and image analysis of the complexes I and V of the mitochondrial respiratory chain, Electron Microsc. Rev. 1:175-199.
Brink, J., Van Breemen, J.F.L., Keegstra, W. and Van Bruggen, E.F.J. (1989) Computer image analysis of two-dimensional crystals of beef heart NADH:ubiquinone oxidoreductase fragments. I. Comparison of crystal structures in various negative stains, Ultramicroscopy 27:79-90 and Ultramicroscopy 27:334. See its frontcover here.
Brink, J., Booy, F.P and Van Bruggen, E.F.J. (1989) Computer image analysis of two-dimensional crystals of beef heart NADH:ubiquinone oxidoreductase fragments. II. Comparison of frozen hydrated and negatively stained specimens, Ultramicroscopy 27:91-100.
Brink, J. and Chiu, W. (1991) Contrast analysis of cryo-images of n-paraffin recorded at 400 kV out to 2.1 Å resolution. J. Microscopy 161:279-295.
Rez, P., Chiu, W., Weiss, J.K. and Brink, J. (1991) The thickness determination of organic crystals under low dose conditions using electron energy loss spectroscopy. Microsc. Res. Tech. 21:166-170.
Brink, J., Chiu, W. and Dougherty, M. (1992) Computer-controlled spot-scan imaging of crotoxin complex crystals with 400 keV electrons at near-atomic resolution. Ultramicroscopy 46:229-240.
Leapman, R.D., Brink, J. and Chiu, W. (1993) Low-dose thickness measurement of glucose-embedded crystals crystals by electron energy loss spectroscopy and STEM dark-field imaging. Ultramicroscopy 52:157-166.
Brink, J. and Chiu, W. (1994) Applications of a Slow-Scan CCD Camera in Protein Electron Crystallography. J. Struct. Biology 113:23-34. See its frontcover here.
Brink, J. and Tam, M.W. (1996) Processing of electron diffraction patterns acquired on a slow-scan CCD camera. J. Struct. Biology 116:144-150.
Sherman, M.B., Brink, J. and Chiu, W. (1996) Performance of a slow-scan CCD camera for macromolecular imaging in a 400 kV electron cryomicroscope. Micron. 27(2):129-139.
Brink, J., Sherman, M. B., Berriman, J. and Chiu, W. (1998) Evaluation of charging on biological specimens in an electron cryomicroscope. Ultramicroscopy. 72:41-52.
Brink, J., Gross, H., Tittmann, P., Sherman, M. B. and Chiu, W. (1998) Reduction of Charging in Protein Electron Microscopy J. Microscopy. 191(1):67-73.
Frey, W., Brink, J., Schief, Jr., W.R., Chiu, W. and Vogel, V. (1998) Projection Structure of Two-dimensional Streptavidin Crystals coordinated to Metal-Chelated Lipid Monolayers. Biophys. J.. 74(5):2674-2679.
Brink, J., Ludtke, S., Yang, C.-Y., Gu, Z.-W., Wakil, S.J. and Chiu, W. (2002) Quaternary structure of human fatty acid synthase by electron cryomicroscopy. Proc. Nat'l. Acad. Sci. 99(1):138-143.
Ming, D., Kong, Y., Wakil, S.J. Brink, J. and Ma, J. (2002) Domain movements in human fatty acid synthase by quantized elastic deformational model. Proc. Nat'l. Acad. Sci. Accepted for publication.

Proceedings papers

Brink, J., Bijlholt, M.M.C., Lamy, J. and Van Bruggen, E.F.J. (1982) Image analysis of Androctonus australis hemocyanin fraction 1, Life Chemistry Reports, 51-52, Supplement Series Vol. 1, in: Structure and function of invertebrate respiratory proteins (E.J. Wood ed.) Harwood Academic Publishers, London, New York.

Boekema, E.J., Booy, F.P., Van Breemen, J.F.L., Brink, J., Keegstra, W. and Schmidt, D.G. (1986) Cryo-EM of various biomacromolecular assemblies, Proc. 11th Int. Cong. EM, Kyoto 3:2423-2424.

Van Bruggen, E.F.J., Wullink, W., Brink, J., Wichertjes, T., Van Breemen, J.F.L., Vonck, J., Oostergetel, G.T. and Keegstra, W. (1988) Possibilities of electron microscopy for the study of biomacromolecules, Proc. 4th Asian Pacific Conf and on EM, (Bangkok) 419-424.

Brink, J. and Chiu, W. (1989) Cryomicroscopy of crotoxin complex crystals at 400 kV, Proc. 47th Ann. Meet. EMSA, 826-827.

Brink, J. and Chiu, W. (1990) Analysis of image contrast in high-resolution images of n-paraffin recorded at 400 kV and -167^oC, Proc. XIIth Int'l Congress for Elect. Microsc. (Seattle), San Francisco Press, 1:86-87.

Brink, J. and Chiu, W. (1992) Recording of 3D electron diffraction intensities of glucose-embedded crotoxin complex crystals at 400 kV using a slow-scan 1024x1024 CCD camera. Proc. Xth. Eur. Congress on EM. (Granada), Secretariado de Publicaciones de la Universidad de Granada, 3:15-16.

Chiu, W., Brink, J., Soejima, T. and Schmid, M.F. (1992) Protein electron crystallography by 400 kV cryo-microscopy. Proc. 50th. Ann. Meet. EMSA, 1054-1055.

Leapman, R., Brink, J. and Chiu, W. (1993) Thickness measurement of glucose-embedded crotoxin complex crystals by EELS. Proc. 51st Ann. Meet. EMSA, 686-687.

Chiu, W., Sherman, M. and Brink, J. (1994) Spot-scan Imaging of ice-embedded catalase crystal on a slow-scan CCD camera in a 400-kV electron cryomicroscope. Proc. 52nd Ann. Meet. MSA, 98-99.

Brink, J. and Chiu, W. (1994) Merging of Electron Diffraction Intensities acquired with a Slow-Scan CCD Camera of Crotoxin Complex Crystals to 3.5 Å. Proc. 52nd Ann. Meet. MSA, 104-105.

Brink, J., Sherman, M., Berriman, J. and Chiu, W. (1994) Charging Phenomena observed on biological specimens in a 400 kV electron cryo-microscope. Proc. 52nd Ann. Meet. MSA, 118-119.

Brink, J., Sherman, M.B. and Chiu, W. (1995) Application of a slow-scan CCD camera in protein electron crystallography. Proc. 53rd Ann. Meet. MSA, 8-9.

Brink, J., Gross, H., Sherman, M.B. and Chiu, W. (1996) Reduction of charging on biological specimens in high-resolution cryo-microscopy. Proc. XIth Eur. Cong. Microsc., Dublin.

Schmid, M.F., Sherman, M.B., Brink, J., Jakana, J., Kelsey, M., Dougherty, M. and Chiu, W. (1997) Exploring macromolecular interactions at high resolution using electron cryomicroscopy at NCMI. Proc. 55th Ann. Meet. MSA, 279-280.

Sherman, M.B., Brink, J. and Chiu, W. (1997) Reduction of charging in biological electron cryomicroscopy Proc. 55th Ann. Meet. MSA, 365-366.

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Last update: April 15th 1998