Electron crystallography and cryomicroscopy of macromolecular assemblies




Who and where:

- Jaap Brink, Ph.D.
- W.M. Keck Center for Computational Biology and Verna and Marrs McLean Department of Biochemistry, Rm. 326B, Baylor College of Medicine, 1 Baylor Plaza, Houston TX 77030, U.S.A.
- tel: +1 (713) 798-6989
- fax: +1 (713) 796-9438
- My geek code: Version: 3.1 GS d- s++: a+ C++++ LI++ P--- L+++ E W+++ N++ o+ K? w--- O! M V-->
PS+ PE Y-- PGP- t++@ 5 X- R- tv! b+ DI++ D@ G- e++++ h---- r++ y+++
For all your comments/suggestions on this and following pages, direct your e-mail to jbrink@bcm.tmc.edu

Research interests:

My research interest deals with the determination of the structure-function relationship of proteins using electron crystallography at the National Center for Macromolecular Imaging at the Baylor College of Medicine in Houston, Texas headed by Prof. Wah Chiu. The NCMI is an NIH supported resource center.

My work focuses currently on the following topics:

- charging and its effects on high-resolution electron cryo-microscopy
- 3D reconstruction of the human Fatty Acid Synthase
- Telemicroscopy
- crystallization of a variety of proteins using the lipid monolayer technique
- slow-scan CCD camera utilization for on-line assessment of specimen quality for data collection

Obtainable Software Packages 

EDP -- GUI-based program for manual indexing and off-line processing of electron diffraction patterns. (archive | tutorial

AUTO -- Suite of FORTRAN 77-programs for completely automated indexing and processing of electron diffraction patterns. (archive | tutorial

PC2MRC -- Tool for converting SCANSALOT-format image files to MRC-format image files. (archive

CONVGATAN -- Tool for converting Gatan-format image files to MRC-format image files. (archive

Selected Papers:

- Brink, J. and Chiu, W. (1994) Applications of a Slow-Scan CCD Camera in Protein Electron Crystallography. J. Struct. Biology, 113:23-34.
- Brink, J. and Tam, M.W. (1996) Processing of electron diffraction patterns acquired on a slow-scan CCD camera. J. Struct. Biology, 116:144-150.
- Sherman, M.B., Brink, J. and Chiu, W. (1996) Performance of a slow-scan CCD camera for macromolecular imaging in a 400 kV electron cryomicroscope. Micron, 27(2):129-139.
- Brink, J., Sherman, M.B., Berriman, J. and Chiu, W. (1998) Evaluation of charging on macromolecules in electron cryomicroscopy. Ultramicroscopy, 72:41-52.
- Brink, J., Gross, H., Tittmann, P., Sherman, M.B. and Chiu, W. (1998) Reduction of charging in protein electron cryomicroscopy. J. Microsc. In press.
- Frey, W., Brink, J., Schief, Jr., W.R., Chiu, W. and Vogel, V. (1998) Projection structure of two-dimensional streptavidin crystals coordinated to metal-chelated lipid monolayers. Biophys. J., 74(5):2674-2679.
-Brink. J., Ludtke, S.J., Yang, C.-Y., Gu, Z.-W., Wakil, S.J. and Chiu, W. (2002) Quaternary structure of human fatty acid synthase by electron cryomicroscopy. Proc. Natl. Acad. Sci. USA, 99(1):138-143.
All papers published so far are here.

Other links:

Misc. images:

- Advisory 2001 PPT file
- actin bundle
- Glu synthase
- sensory rhodopsin II

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